OneG


Prediction of cryptic intermediates in the unfolding kinetics of proteins under native conditions


I. Calculation of ΔGHX for the given protein.


Enter the PDB ID:
(OR)
Upload the PDB file:

(OR)

Enter the amino acid sequence and related structural information.

Sequence:
Position of cysteine residue(s). (Help)
Position of cis proline(s). (Help)


pH:
Temperature: K
Solvent: H2O D2O Others

Ionic Strength: Low High

Enter the reference rates if the solvent is other than D2O.

ka kb kw

Do you want to account for the effect of pKR of amino acids in the calculation ?

Yes No

Upload the file containing kex values (in min-1) of amino acids.


(OR)

Upload ΔGHX of the residues.


Updated on May 8, 2015
for queries contact:
sivaram @ scbt.sastra.edu
tricha @ bioinfo.sastra.edu
Please cite the website and the following reference(s) in your publication(s) if you use the OneG.
1. T. Richa and T. Sivaraman. OneG: A computational tool for predicting cryptic intermediates in the unfolding kinetics of proteins under native conditions, PLoS ONE (2012), 7(3): e32465.
2. T. Richa, Computational tools for predicting cryptic intermediates and metastable states in the unfolding kinetics of proteins under native conditions. Ph.D. Dissertation (2014), SASTRA University, Tamil Nadu, India.