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Validating the cryptic intermediates detected by experimental methods.

ΔGU: kcal/mol
mu: kcal/mol/M
Temperature: K
ΔGHX: kcal/mol
mx**: kcal/mol/M
No. of cryptic intermediaiates:

**Data preprations
ΔGU = mu · Cm
ΔGHX = mx· Cm

ΔGU – Free energy of unfolding determined by optical methods.
mu – Cooperative unfolding constant.
Cm – Denaturant concentration wherein ΔGU is zero.
ΔGHX – Free energy of exchange (averaged out to two largest residue-specific ΔGHX) of proteins in the absence of denaturant.
mx – Cooperative constant of global unfolding residues.

Updated on January 2, 2015
for queries contact:
sivaram @
tricha @
Please cite the website and the following reference(s) in your publication(s) if you use OneG–Vali.
1. T. Richa and T. Sivaraman. OneG–Vali: A Webserver for detecting, estimating and validating cryptic intermediates of proteins under native conditions. RSC Adv. (2014), 4(68):36325-36335.
2. T. Richa, Computational tools for predicting cryptic intermediates and metastable states in the unfolding kinetics of proteins under native conditions. Ph.D. Dissertation (2014), SASTRA University, Tamil Nadu, India.