ENTER/UPLOAD THE PDB FILE
Ex. : 2CRT
Note: Make sure the residue number of the PDB file starts with '1' and ends with 'n' without missing numbers.Also make sure residues represented in 'SEQRES' and in atomic coordinates are identical to each other.
ENTER FREE ENERGY OF UNFOLDING (CORRECTED TO PROLINE EFFECT) FOR THE GIVEN PROTEIN
Ex: 5.5 (in kcal/mol)
UPLOAD THE FILE CONTAINING RESIDUE-SPECIFIC ΔGHX OF THE PROTEIN
DOWNLOAD THE PYMOL FILE
Open the protein PDB file in pymol.
File->Resume->Select your File